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Thermo Fisher cell proliferation

<t>Proliferation</t> of minced cartilage and isolated cells on day 28. A biopsy from a chondral allograft, minced cartilage minced manually or arthroscopically and isolated chondrons and chondrocytes were cultured in fibrin gel and proliferation was determined on day 28. (a) Graph showing the percentage of proliferated cells in minced cartilage and isolated cells on day 28. Each point represents a donor with a minimum of one replicate per donor. Each donor is represented by a different symbol. Standard deviations are shown. A GEE analysis with a Bonferroni post-hoc correction for multiple testing between all groups was performed. All statistical significance for comparisons between groups including specific p-values and 95%-CI can be found in the appendix. Statistical differences are shown and marked as following: The chondrocytes group was statistically significantly higher than the arthroscopically and manually minced group. Picture b through f shows proliferated cells (green) and all cells (blue). (b) Chondral allograft biopsy; (c) Manually minced cartilage; (d) Arthroscopically minced cartilage; (e) Chondrons; (f) Chondrocytes. Isolated cell groups show a homogenous distribution of proliferated cells. Minced cartilage groups show less proliferation on the edges in comparison to the chondral allograft biopsy. All pictures taken at 20x magnification.

Cell Proliferation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ethynyl/5'-Ethynyl-2'-deoxyuridine/pmc13320028-144-2-10
Average 99 stars, based on 1 article reviews

cell proliferation - by Bioz Stars, 2026-09

99/100 stars

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1) Product Images from "Arthroscopically and manually minced cartilage demonstrates lower cell viability and lower proteoglycan deposition compared to isolated chondrons and chondrocytes"

Article Title: Arthroscopically and manually minced cartilage demonstrates lower cell viability and lower proteoglycan deposition compared to isolated chondrons and chondrocytes

Journal: Osteoarthritis and Cartilage Open

doi: 10.1016/j.ocarto.2026.100843

Proliferation of minced cartilage and isolated cells on day 28. A biopsy from a chondral allograft, minced cartilage minced manually or arthroscopically and isolated chondrons and chondrocytes were cultured in fibrin gel and proliferation was determined on day 28. (a) Graph showing the percentage of proliferated cells in minced cartilage and isolated cells on day 28. Each point represents a donor with a minimum of one replicate per donor. Each donor is represented by a different symbol. Standard deviations are shown. A GEE analysis with a Bonferroni post-hoc correction for multiple testing between all groups was performed. All statistical significance for comparisons between groups including specific p-values and 95%-CI can be found in the appendix. Statistical differences are shown and marked as following: The chondrocytes group was statistically significantly higher than the arthroscopically and manually minced group. Picture b through f shows proliferated cells (green) and all cells (blue). (b) Chondral allograft biopsy; (c) Manually minced cartilage; (d) Arthroscopically minced cartilage; (e) Chondrons; (f) Chondrocytes. Isolated cell groups show a homogenous distribution of proliferated cells. Minced cartilage groups show less proliferation on the edges in comparison to the chondral allograft biopsy. All pictures taken at 20x magnification.

Figure Legend Snippet: Proliferation of minced cartilage and isolated cells on day 28. A biopsy from a chondral allograft, minced cartilage minced manually or arthroscopically and isolated chondrons and chondrocytes were cultured in fibrin gel and proliferation was determined on day 28. (a) Graph showing the percentage of proliferated cells in minced cartilage and isolated cells on day 28. Each point represents a donor with a minimum of one replicate per donor. Each donor is represented by a different symbol. Standard deviations are shown. A GEE analysis with a Bonferroni post-hoc correction for multiple testing between all groups was performed. All statistical significance for comparisons between groups including specific p-values and 95%-CI can be found in the appendix. Statistical differences are shown and marked as following: The chondrocytes group was statistically significantly higher than the arthroscopically and manually minced group. Picture b through f shows proliferated cells (green) and all cells (blue). (b) Chondral allograft biopsy; (c) Manually minced cartilage; (d) Arthroscopically minced cartilage; (e) Chondrons; (f) Chondrocytes. Isolated cell groups show a homogenous distribution of proliferated cells. Minced cartilage groups show less proliferation on the edges in comparison to the chondral allograft biopsy. All pictures taken at 20x magnification.

Techniques Used: Isolation, Cell Culture, Comparison

Injection:

Article Title: Intermittent parathyroid hormone employs autonomous and non-autonomous mechanisms to drive osteogenesis from Ebf3-expressing skeletal progenitor cells
Article Snippet: Antibodies for FACS of human samples included CD235ab (BioLegend, 306614), CD14(BioLegend, 301824), CD45 (BioLegend, 304052), CD31 (BioLegend, 303122). .. To evaluate cell proliferation, 1.5 mg of 5-ethynyl-2’-deoxyuridine (EdU) (Invitrogen, A10044) was dissolved in PBS and administered via intraperitoneal injection to mice 12 hours before sacrifice. ..

Article Title: Wnt Regulates Cellular Differentiation and Retinoid Metabolism Genes in the Mouse Stomach.
Article Snippet: Gastric organoids generated from wild-type C57BL/6 mice (Jackson Labs 118 #000664) were used for bulk RNA sequencing experiments. .. For analysis of proliferation, mice 119 were injected with 5-ethynyl-2’-deoxyuridine (EdU, 25 mg/kg, Invitrogen) 2 hours prior to tissue 120 collection. ..

Article Title: Persistent and transient senescent cells contribute to brain-barrier development.
Article Snippet: .. 5-ethynyl-2’-deoxyuridine (EdU; Invitrogen) and O-propargyl-puromycin (OPP; Invitrogen) incorporation assays were performed by intraperitoneal (IP) injection of pregnant dams with EdU (10mM, Invitrogen) or OPP (50 mg/kg, MedChemExpress) for 1 h prior to embryo collection. .. For tamoxifen labeling experiments, tamoxifen (Sigma, T5648) was prepared in 100% ethanol solution (20 mg/mL) and stored at -20oC.

Article Title: TM4SF1 mediates YAP/TAZ regulation of coronary artery formation.
Article Snippet: .. For cell proliferation analysis, pregnant mice were intraperitoneally injected with 5- ethynyl-2′-deoxyuridine (EdU; Life Technologies) at a dosage of 100 mg/kg. ..

Incubation:

Article Title: Replication stress induced exposure to methotrexate in root meristem cells of Vicia faba.
Article Snippet: .. Root meristems from all experimental series were incubated with 10 μM 5-ethynyl-2′-deoxyuridine (EdU; Thermo Fisher Scientific, Warsaw, Poland) under darkened conditions. .. After 30 min, the dissected meristems were fixed in 4% phosphate buffered saline (PBS)–buffered paraformaldehyde (4 °C; 20 min; pH 7.4; Merck Life Science, Poznan, Poland) and then macerated with 2.5% citrate-buffered pectinase (pH 5.0; 30 min; Merck Life Science, Poznan, Poland).

Article Title: Limitations of the p16-3MR mouse model for detecting and eliminating senescent cells.
Article Snippet: .. For proliferation assays, MEFs were incubated with 2 μM 5-ethynyl-2′-deoxyuridine (EdU) for 3 days, fixed with 4% paraformaldehyde (PFA), and stained using the Click-iT EdU Cell Proliferation Kit for Imaging (C10640; Invitrogen). .. For cell viability assays, MEFs were treated with 10 μg/mL GCV or vehicle (Dimethyl sulfoxide (DMSO; 13445-74, Nacalai tesque)) for 6 days, © The Author(s) EMBO reports 11 with medium replaced every 2 days.

Staining:

Imaging:

Article Title: Transcriptional control of gemma dormancy via dual pathways in Marchantia polymorpha.
Article Snippet: UN CO RR EC TE D MA NU SC RI PT

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